EZ1 DNA Cards

For easy setup of protocols using EZ1 DNA Blood Kits and the EZ1 DNA Tissue Kit on the BioRobot EZ1

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EZ1 DNA Blood Card

Cat. No. / ID: 9015585

Pre-programmed card for BioRobot EZ1 DNA Blood 200 µl and 350 µl Protocols

Sample type

Blood

Dried Blood

Tissue

Buccal Swab

Buffy Coat

Paraffin Section

Bacteria

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EZ1 Cards are used in combination with EZ1 Kits on the BioRobot EZ1. Please refer to the specific EZ1 Kit handbook and the BioRobot EZ1 User Manual for information about product use.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Easy protocol setup with credit-card ease of use
Standardized processing ensures reproducible results
Optimized protocols help to ensure reliable purification
Automated purification of genomic DNA

Product Details

EZ1 DNA Cards are preprogrammed with protocols for purification of DNA from blood and blood-derived samples, dried blood, tissues, buffy coat, paraffin-embedded tissues, buccal swabs, human samples, swabs, biopsies and bacterial cultures.

Performance

The yield and quality of the purified DNA are suited for use in many applications (see figures "Representative yields of high-purity DNA from buffy coat samples", "Consistent PCR results", "Reproducible performance in sensitive PCR analysis" and "Sensitive PCR using DNA purified from preserved blood", and also table "DNA yields obtained from bacterial suspension cultures").

DNA yields obtained from bacterial suspension cultures

Sample type	Gram	Optical density (OD) value	Starting volume	DNA yield (µg)
Escherichia coli	–	0.7	200 µl	6.6 ± 0.4
Pseudomonas spp.	–	0.7	200 µl	9.0 ± 0.5
Bacillus subtilis	+	0.4	1000 µl*	5.7 ± 0.2
Staphylococcus spp.	+	0.2	1000 µl*	5.7 ± 0.2

* Bacteria cultures were pelleted and resuspended in PBS.

See figures

Representative yields of high-purity DNA from buffy coat samples.

Consistent PCR results.

Reproducible performance in sensitive PCR analysis.

Sensitive PCR using DNA purified from preserved blood.

Principle

EZ1 Cards specify a choice of purification protocols without any manual data entry. Additional EZ1 Cards may be purchased at any time to increase the application range of the BioRobot EZ1.

Procedure

Simply insert the EZ1 Card with the desired protocol into the EZ1 Card slot and start the instrument.

Applications

The high-quality DNA obtained using the BioRobot EZ1 and EZ1 DNA Cards with EZ1 DNA Blood Kits and the EZ1 DNA Tissue Kit is suitable for use in a wide range of applications, such as:

Genotyping analysis, including STR, SNP, VNTR, RAPD, NASBA and AFLP technologies
Population genetics and pharmacogenomic studies
Oncology and infectious disease research
Purification of DNA from archived samples
PCR and real-time RT-PCR
Molecular testing research
Sequencing
Bacterial detection research
Epidemiology and infection control
Quantification and resistance profiling by PCR
Forensic research

Features	Specifications
Applications	PCR, real-time RT-PCR, genotyping
For automated processing	BioRobot EZ1
Processing	Automated

Supporting data and figures

Representative yields of high-purity DNA from buffy coat samples.

Each data point represents the average yield from 3 replicates.

Resources

For use with EZ1 instruments

EZ1&2 DNA Tissue Kit
For automated purification of DNA from tissue and other samples using EZ1 instruments

EZ1&2 DNA Blood 200 μl Kit
EZ1&2 DNA Blood 350 μl Kit
For automated purification of DNA from blood and buffy coat using EZ1 instruments

FAQ

Yes, please follow the Supplementary Protocol 'Purification of genomic DNA from whole blood, optimized for use in MRC-Holland MLPA® assays, using EZ1® DNA Blood Kits’.

FAQ ID -1169

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

Yes, please follow the Supplementary Protocol 'Isolation of DNA from cultured cells using the EZ1 DNA Tissue Kit' (MA15).

FAQ ID -990

Making consistent buffy samples takes practice. Serum and erythrocytes are easily isolated alongside the 10x concentrated leukocyte fraction. Our Supplementary Protocol 'Isolation of genomic DNA from buffy coat using the EZ1 DNA Blood 350 µl Kit' (MA02) contains instructions and recommendations for preparation of buffy coat for DNA isolation on the BioRobot EZ1.

FAQ ID -1249

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

Yes, please check the EZ1 DNA Tissue Handbook for instructions on pretreatment and extraction protocol.

FAQ ID -1260

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

Yes, please follow the Supplementary Protocol 'Purification of DNA from fungi using the EZ1 DNA Tissue Kit' (MA35).

FAQ ID -992

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246