Products
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Not available in the USA.
Features
- High reliability using the internal control
- Sensitive detection of HCV RNA in plasma
- Quantitation of viral copies over a very broad range
Product Details
The artus HCV RG RT-PCR Kit and the artus HCV QS-RGQ Kit are ready-to-use molecular detection kits for real-time RT-PCR on Rotor-Gene Q instruments. The kits provide all necessary reagents for HCV RNA detection and quantitation. The artus HCV RG RT-PCR Kit uses manual sample preparation and assay setup techniques. The artus HCV QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
Principle
The artus HCV RG RT-PCR Kit and the artus HCV QS-RGQ Kit are based on the amplification and simultaneous detection of a specific region of the HCV genome using real-time RT-PCR. The kits provide high levels of analytical specificity and sensitivity over a broad linear range.
Each artus HCV Kit provides 4 HCV quantitation standards (see figure " Reliable quantitation of HCV copies"). Use of the standards enables accurate quantitation of viral copies. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR.
Each artus HCV Kit provides 4 HCV quantitation standards (see figure " Reliable quantitation of HCV copies"). Use of the standards enables accurate quantitation of viral copies. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR.
See figures
Procedure
artus HCV RT-PCR Kits provide all necessary reagents optimized for reliable HCV RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
Complete automated system from sample to HCV detection
The QIAsymphony RGQ workflow solution for HCV detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus HCV QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable detection of HCV RNA with a complete workflow for research applications (see figure " Integrated QIAsymphony RGQ system for HCV detection").
See figures
Applications
The artus HCV RG RT-PCR Kit enables rapid and sensitive detection and quantitation of HCV RNA purified from plasma using the QIAamp DSP Virus Kit for research applications.
The artus HCV QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete workflow from sample to HCV RNA detection and quantitation for research applications.
Supporting data and figures
Reliable quantitation of HCV copies.
Reliable quantitation of HCV copies.
Resources
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)