QIAamp DSP DNA Mini Kit

For purification of genomic, mitochondrial, bacterial, parasite, or viral DNA

Features

Rapid purification of high-quality DNA
Consistent, high yields
Removal of contaminants and inhibitors

Product Details

The QIAamp DSP DNA Mini Kit provides silica-membrane–based nucleic acid purification from tissues, swabs, CSF, blood, body fluids, or washed cells from urine. The spin-column procedure does not require mechanical homogenization, so total hands-on preparation time is only 20 minutes. The QIAamp DSP DNA Mini Kit is for in vitro diagnostic use. The procedure can be fully automated on the QIAcube.

Performance

The QIAamp DSP DNA Mini Kit uses QIAamp Mini spin columns for purification of high-quality DNA from tissues, swabs, CSF, blood, bone marrow, body fluids, lymphocytes, and washed cells from urine. Yields and concentration of DNA depend on sample type and elution volume.

Principle

The QIAamp DSP DNA Mini Kit provides fast and easy methods for purification of total DNA. No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. The QIAamp DSP DNA Mini Kit provides purified DNA ready for use in downstream applications in just 20 minutes.

Procedure

The QIAamp DSP DNA Mini Kit procedure, using QIAamp Mini spin columns, has 4 simple steps: lyse, bind, wash twice, and elute.

Purification of DNA using the QIAamp DSP DNA Mini Kit can be automated on the QIAcube. The QIAamp DSP DNA Mini Kit includes QIAamp Mini spin columns, which can be processed using a centrifuge or a vacuum manifold (i.e., QIAvac 24 Plus with VacConnectors and VacValves). If automating the QIAamp DSP DNA Mini Kit on the QIAcube instrument, the instrument may process fewer than 50 samples due to dead volumes, evaporation, and additional reagent consumption by automated pipetting. QIAGEN guarantees 50 sample preps with manual use of the QIAamp DSP DNA Mini Kit.

Applications

The QIAamp DSP DNA Mini Kit provides silica-based nucleic acid purification from the following sample types:

Tissues, swabs, CSF
Whole blood, plasma, serum, or buffy coat
Bone marrow
Body fluids, lymphocytes
Washed cells from urine

Resources

FAQ

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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