EZ1 Advanced XL

For automated purification of nucleic acids from up to 14 human, forensic, or molecular diagnostics samples

S_1163_IAS_EZ1_0115

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✓ Knowledgeable and professional Product & Technical Support

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EZ1 Advanced XL, Priority Package

Cat. No. / ID:   9001875

Instrument and service agreement package: robotic workstation for automated purification of nucleic acids from up to fourteen samples using EZ1 Kits; includes installation, application training, Full Agreement for two years with a two business day response time and two preventive maintenance visits.
InstrumentAccessories
EZ1 Advanced XL
Silicone Grease, BR M48 & BR EZ1
Service optionService Options
Priority Package
System
Priority Package Plus
System PRV-2
This product will be discontinued as of December 31, 2024 or until stocks last.
EZ1 Advanced Instruments are intended to be used only in combination with QIAGEN kits indicated for use with EZ1 Advanced Instruments for the applications described in the kit handbooks.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • High-quality genomic DNA and RNA from a range of sample materials
  • Viral RNA/DNA and bacterial DNA from biomedical samples
  • Throughput of up to 14 samples per run
  • Effortless data management with full traceability
  • UV lamp to help minimize contamination risk

Product Details

The EZ1 Advanced XL performs automated nucleic acid purification for a wide range of sample types relevant for molecular diagnostics, human identity testing, forensics, biomedical research, and gene expression analysis.

Find more information on EZ1 Kits and EZ1 Cards.

Performance

Standardization and traceability

The EZ1 Advanced XL sets new standards in data management. Barcode reading enables complete tracking of samples and reagents throughout the entire purification process. Notes and assay identification information can also be easily entered. For increased process control and reliability, reagent identification and lot numbers are logged together with the expiration date, and a warning is issued if the expiration date has been exceeded. The run report, automatically generated at the end of each protocol, contains all relevant instrument information, including maintenance procedures and decontamination data. The instruments generate a pdf and a csv file after each run. Data can be easily transferred to a printer or computer within the network and the report file can be subsequently processed by a LIMS (Laboratory Information Management System).

Proven technology with improved performance

Proven EZ1 Kits, in combination with EZ1 Advanced XL Cards, ensure high-performance results in purification procedures. The unrivaled ease of use of the EZ1 Advanced XL enables fast and reproducible purification, without the need for specialized training or previous experience with laboratory automation. The extensive application range, including DNA purification from blood, tissues, and human identity and forensic samples, RNA purification from cells and tissues, and purification of viral nucleic acids from a range of sample types, delivers the flexibility that is essential for laboratories.

Safety and reliability

The EZ1 Advanced XL has been designed to ensure high process safety, high performance, and user convenience. A sensor ensures that the instrument door remains closed during sample preparation, reducing contact of laboratory personnel with potentially infectious samples and protecting sample integrity. At the end of each run, an intelligent-design UV lamp decontaminates the inner surface of the instrument. UV decontamination is highly effective in eliminating Gram-positive and Gram-negative bacteria (see tables). If required, the instrument can be placed in a safety cabinet, providing increased user safety.

E. coli colony numbers
Irradiation (min)Run

1

2

3

4

5

6

301000000
302000100
3031000003
30Control311352367367351396
601000000
602000000
603000000
60Control348307326326396385
S. haemolyticus colony numbers
Irradiation (min)Run

1

2

3

4

5

6

301121017264
302000000
30310021103
30Control248256396314321267
601000000
602000000
6031000000
60Control264305368292347388

Principle

Automated sample preparation enables efficient, standardized purification of nucleic acids. Manual sample handling is minimized, ensuring safety to the user and reliable processing of samples.

The EZ1 Advanced XL instrument uses proven EZ1 Kits for purification of DNA, RNA, and viral nucleic acids from a wide range of starting materials. Purified nucleic acids are ready for immediate use in downstream applications. The combination of an easy-to-use instrument, with error-free protocol selection and worktable setup, and prefilled, sealed reagent cartridges makes nucleic acid purification easy.

Procedure

Flexibility and ease of use

The self-contained EZ1 Advanced XL instrument ensures optimal ease of use and walkaway automation. A wide range of protocols is available for purification of DNA, RNA, or viral nucleic acids. Up to 14 samples can be processed per run in as little as 20 minutes. The instrument is operated via a keypad, and instrument information is displayed on the VFD (vacuum fluorescent display).

All processing steps are performed by the instruments — from piercing reagent cartridges to elution of pure nucleic acids. A separate computer is not required for operation, and EZ1 Kits include all reagents and accessories required to process your samples. EZ1 Kits provide prefilled, foil-sealed reagent cartridges that remain sealed until the instrument door is closed and the protocol run started, reducing the risk of contamination during setup.

If desired, up to 4 EZ1 Advanced XL instruments can be connected to an external computer, expanding throughput up to 56 samples per run. This enables you to easily adapt your laboratory setup to changing sample preparation requirements.

EZ1 Kits and EZ1 Advanced XL Cards for a wide range of applications

The EZ1 Advanced XL uses proven EZ1 Kits with preprogrammed protocols on EZ1 Advanced XL Cards. EZ1 Kits are available for a broad range of applications including work in human identity and forensics, biomedical research, and gene expression analysis (see table).

Combinations of EZ1 Advanced XL Cards and EZ1 Kits
Card Kit Sample
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 200 µl Kit Blood and blood-derived samples
EZ1 Advanced XL DNA Blood Card EZ1 DNA Blood 350 µl Kit Blood and blood-derived samples
EZ1 Advanced XL DNA Dried Blood Card EZ1 DNA Tissue Kit Dried blood
EZ1 Advanced XL DNA Buffy Coat Card EZ1 DNA Blood 350 µl Kit Buffy coat
EZ1 Advanced XL DNA Tissue Card EZ1 DNA Tissue Kit Tissues
EZ1 Advanced XL DNA Paraffin Section Card EZ1 DNA Tissue Kit Paraffin-embedded tissues
EZ1 Advanced XL DNA Buccal Swab Card EZ1 DNA Tissue Kit Buccal swabs
EZ1 Advanced XL DNA Investigator Card EZ1 DNA Investigator Kit Forensic and human identity samples
EZ1 Advanced XL DNA Bacteria Card EZ1 DNA Tissue Kit Human samples, swabs, biopsies, bacterial cultures
EZ1 Advanced XL Virus Card v2.0 EZ1 Virus Mini Kit v2.0 Viral DNA and RNA and bacterial DNA from serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swab samples
EZ1 Advanced XL RNA Card EZ1 RNA Universal Tissue Kit Any type of human or animal tissue
EZ1 Advanced XL DSP DNA Blood Card* EZ1 DSP DNA Blood Kit* Human whole blood
EZ1 Advanced XL DSP Virus Card* EZ1 DSP Virus Kit* Viral DNA and RNA and bacterial DNA from serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swab samples

Applications

Nucleic acids purified using the EZ1 Advanced XL are ready for use in a wide range of downstream applications, including:

  • Oncology, forensics, and genetic identity testing
  • Genotyping
  • Infectious disease research, including bacterial genotyping
  • Gene expression analysis

Services

Have your throughput demands increased? Do you want to expand your application range or further streamline workflows? Do you need to manage more complex samples? Do you need improved connectivity?

If the answer to any of these questions is yes, then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply exchange an old instrument for a new one. Improve your efficiency by trading in a competitor instrument or trading up with an older QIAGEN instrument.

Learn more about trade-in/trade-up opportunities.

Supporting data and figures

Specifications

FeaturesSpecifications
FeaturesTouch LCD user interface, IC card with protocols/software, barcode reader, UV lamp
Pipetting range50-1000 µl
Kits designed for this instrumentQIAGEN EZ1 Kits
TechnologyMagnetic-particle technology
Instrument dimensions510 x 510 x 570 mm (w x d x h)
Technical data of the instrumentWeight 48 kg, 100–240 V AC, 50–60 Hz
Protocols/main application on this instrumentPurification of DNA, mRNA, total RNA, and viral RNA and DNA
Samples per run (throughput)1–14 samples per run

Service Plans

EZ1, Full Agreement

Cat. No. / ID:   9237721

On-site EZ1 Advanced or EZ1 Advanced XL instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.

Resources

Brochures & Guides (5)
Explore our new, easy-to-navigate digital Product Profile!
Advanced sample collection, automation and STR solutions for kinship testing

Streamline your workflow with QIAGEN solutions for human identity and forensic testing

Supplementary Protocols (4)
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL
Supplementary protocol using the Flip Cap Rack and the EZ1 Advanced XL Flip Cap Card.
Safety Data Sheets (1)
Validation Reports (1)
Certificates of Analysis (1)

FAQ

What to do if I get error 24 (Open door in motion) on my EZ1 instruments?

Make sure a correct protocol card is inserted for the corresponding instrument. Protocol cards for BioRobot EZ1, EZ1 Advanced, and EZ1 Advanced XL cannot be interchanged. If you use an incorrect protocol card, you can get error 24.

If you are using a correct protocol card, please contact QIAGEN Technical Service.

FAQ ID -9128
What is the lifetime of the UV lamp on an EZ1 Advanced and EZ1 Advanced XL?

The lifetime of the UV lamps is 1200 cycles on an EZ1 Advanced.

The lifetime of the UV lamps is 1500 cycles on an EZ1 Advanced XL.

FAQ ID -9129
I observe more than normal magnetic bead carryover in my eluates after extraction on EZ1 instruments. What should I do?
  1. Make sure the maximum recommended sample amount is not exceeded.
  2. When using blood samples, make sure no clots are present.
  3. Make sure instrument is connected to a UPS (Uninterrupted Power Supply) to avoid power surge.
  4. Be sure to lubricate the O-rings of the tip adapters on the plunger unit, every week with the silicone grease (cat. no. 9239334). Refer to the user manual for instructions. Check that the tip adapters do not have excess grease.
  5. Perform a pipetting accuracy test using the appropriate Test Card for your instrument: EZ1 Test Card (cat. no. 9016187), EZ1 Advanced Test Card (cat. no. 9018300), or EZ1 Advanced XL Test Card (cat. no. 9018706).
  6. Teaching may have become corrupted. Check teaching. For instructions contact QIAGEN Technical Service.

If the issue persists, please contact QIAGEN Technical Service.

FAQ ID -9126
What to do if I get error 29 (Backup memory on the CPU is destroyed) on my EZ1 instrument?

          Do not remove protocol cards from the instrument while the instrument is switched on.

If the error 29 occurred on a BioRobot EZ1, you will need to clear the protocol memory and perform teaching. For instructions and teaching data sheet, contact QIAGEN Technical Service.

If the error 29 occurs on an EZ1 Advanced or an EZ1 Advanced XL, restart the instrument. The error should clear. If the error persists, contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

 

FAQ ID -9122
What to do if I get error 25 (Abnormal input from bottom sensor in motion) on my EZ1 instrument?

Check that the recommended silicone grease (cat. no. 9239334) is used to lubricate the tip adapters on the plunger unit.

Make sure the tip adapters do not have excess grease. Wipe off any excess. Turn off the instrument and perform a test run to see if the error persists.

If correct grease was used and excess grease is not an issue, please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.



FAQ ID -9121
I get “Master Data Error” after turning on my EZ1 instrument? What should I do?

Instrument seems to have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

If issue persists, a service visit may be needed.

FAQ ID -9127
What is the part number for transport lock or knurled knob for packing the EZ1 Advanced XL instrument?

 

Part number for transport lock for EZ1 Advanced XL is 9019723. You can place an order by calling your local QIAGEN customer care

FAQ ID -9125
What to do if I get error 13 (Failed to return to P axis, protocol in run) on my EZ1 instrument?

Instrument may have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

If issue persists, a service visit may be needed.

FAQ ID -9130
What to do if I get error 14 (Failed to return to M axis, protocol in run) on my EZ1 instrument?

Instrument may have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

If issue persists, a service visit may be needed.

FAQ ID -9132
What to do if I get error 19 (Y axis end limit, protocol in run) on my EZ1 instrument?

Check the workstation for any obstructions or any incorrect placement of the consumables (samples tubes, elution tubes, reagent cartridge, or tips).

If the issue persists, please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument.

FAQ ID -9120
I get “UV lamp did not ignite error” on my EZ1 Advanced and EZ1 Adavanced XL. What should I do?

UV lamp needs to be replaced. Please contact QIAGEN Technical Service to schedule service visit.

FAQ ID -9131
What to do if I get error 0182 (Attempt to move beyond the z-m axis, interference prevention value) on my EZ1 instrument?

 

Instrument may have lost teaching. Please contact QIAGEN Technical Service to obtain teaching instructions and teaching data sheet for your instrument. If teaching does not resolve the issue, a service visit may be needed.

FAQ ID -9123
What is the part number for silicone grease for greasing the O-ring of tip adapters on EZ1 series of instruments?

 The catalog number for Silicone grease for O-rings of Geno M6, M48, and EZ1 BioRobots is # 9239334. You can place an order by calling your local QIAGEN customer care.

FAQ ID -9124