3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?

Yes, please visit our website section 'Using endogenous control genes in real-time RT-PCR' for general information. It provides a list of relative gene expression levels for commonly used human and mouse housekeeping genes.

QuantiTect Primer Assays are primer sets for use in SYBR Green based real-time RT-PCR analysis of housekeeping genes.

 

In addition, you may want to refer to the following citation on reference gene selection for quantitative real-time PCR:

• Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A., 2004.  Guideline to reference gene selection for quantitative real-time PCR.  Biochem Biophys Res Commun. 313(4): 856-62.