3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?

After reconstitution of the lyophilized primers in 550ul nuclease-free water or TE, the miScript Primer Assay and miScript Precursor Assay 100 reaction tubes will be at 10X stock concentration.  They should be used at 1X concentration (0.5 uM) in the PCR/RT-PCR reaction (eg. for a 25ul reaction, 1/10^th of the total volume, or 2.5ul, should be primer). For Fluidigm^® BioMark^TM System related applications, please refer to the miScript Microfluidics Handbook for primer concentrations and reconstitution recommendations.