Cell Line Species/Tissue: |
Human
/
|
Transfection Reagent: |
HiPerFect HTS |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
96-well |
Number of Cells:
|
5*10e3 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
2,5nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
0,25µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
Knockdown Efficiency (%): |
95% |
|
Any modifications to the protocol?:
|
|
Notes: |
|
References: |
|