Transfection Cell Database - Detailed View


Cell Line: PC-3

Cell Line Species/Tissue: Human / Prostate Carcinoma 
Transfection Reagent: Attractene 
Nucleic Acid:
DNA 
Growth Medium:
 
Percent Serum (%):
 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
80% 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
1,0 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
1,5µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
 
 
Any modifications to the protocol?:
 
Notes:
Customer compared Attractene in 3 different prostate cancer cells to FuGeneHD: 22Rv1, LNCaP and PC3 Worked 10x better than FuGene HD. Co- transfection of different plasmids.  
References: