| Cell Line Species/Tissue: |
Drosophila
/
|
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
|
Reporter System:
|
pCoHygro selection vector cotransfected |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
25 cm2 flasks |
Number of Cells:
|
5 000 000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1.8 µg |
Amount of Enhancer (µl):
|
16 µl |
Amount of Reagent (µl):
|
20 µl |
Complex Incubation on Cells (hrs):
|
24 hrs |
Analysis Performed Post-Transfection (hrs):
|
168 hrs |
Transfection Efficiency (%):
|
80-90 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |