| Cell Line Species/Tissue: |
Human
/
cancer cell |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI 1640 |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
no information |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.3 µM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| The Pharmacogenomics Journal (2005) 5, 112-125
Y. Huang, et al.
Correlating gene expression with chemical scaffolds of cytotoxic agents: ellipticines as substrates |