| Cell Line Species/Tissue: |
Human
/
Neuroblastoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
Serum was added 4h after transfection, 10% final conc. |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
18.000 cells/well |
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
5nM, 10nM, 50 nM, 150 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
12µl |
Complex Incubation on Cells (hrs):
|
72h |
Analysis Performed Post-Transfection (hrs):
|
72h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
1%, 33%; 36%; 47% |
| |
| Any modifications to the protocol?:
|
| no serum for the first 4 hours during transfection |
| Notes: |
| RT-PCR (One-step; QIAGEN) |
| References: |
| |