| Cell Line Species/Tissue: |
human
/
epithelioid fibrosarcoma |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DME |
Percent Serum (%):
|
10 |
Reporter System:
|
gene of interest |
Plasmid Purification Method:
|
QIAGEN Plasmid Kits |
Plate Format:
|
60-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
30 |
Amount of Nucleic Acid (µg):
|
5 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
2 |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
36% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Analysis via FACS. |
| References: |
| Rasko, R.J., Gottschalk, R.J., Jue, S.F., and Miller A.D. (1997) Improved transfection efficiency of HT-1080, a fibrosarcoma cell line, using SuperFect Reagent. QIAGEN News 1997 No. 2, 8 |