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Supplemental Microbial qPCR Mastermix (Fluorescein)
Cat. No. / ID: 330540
2 tubes of 1.35 ml each
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The Supplemental Microbial qPCR Mastermix (Fluorescein) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- All-in-one mix for PCR using 5' hydrolysis probe-based detection
- Yields specific products with minimal primer-dimers
- Enables microbial profiling with instruments using fluorescein as a reference dye
Product Details
Supplemental Microbial qPCR Mastermix (Fluorescein) is highly suited for real-time PCR applications using 5' hydrolysis probe-based detection that uses fluorescein as a reference dye, including BioRad iCycler, MyiQ, and iQ5. This supplemental mastermix is for use in automated pipetting systems that require more mastermix in addition to the mastermix supplied with the Microbial DNA qPCR Arrays and Assay Kits. Supplemental Microbial qPCR Mastermix (Fluorescein) contains real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and fluorescein dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate 5' hydrolysis probe results by preventing the amplification of primer-dimers and other nonspecific products.
Principle
Supplemental Microbial qPCR Mastermix (Fluorescein) contains real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and fluorescein dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate 5' hydrolysis probe results by preventing the amplification of primer-dimers and other nonspecific products.
Applications
Supplemental Microbial qPCR Mastermix (Fluorescein) is highly suited for use in automated pipetting systems requiring additional mastermix in excess of the mastermix supplied with the Microbial DNA qPCR Arrays and Assay Kits.
Resources
Safety Data Sheets (1)
Technical Information (1)
Kit Handbooks (1)
Certificates of Analysis (1)
FAQ
What are the storage conditions for the Microbial DNA qPCR products?
Which Microbial qPCR Mastermix should I use?