Transfection Cell Database - Detailed View


Cell Line: Ref52

Cell Line Species/Tissue: Rat / Embryo 
Transfection Reagent: PolyFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
6-well plate 
Number of Cells:
 
Percent Confluence(%):
60-70 
Amount of Nucleic Acid (µg):
1,5 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
4-6 
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
20-40 
 
Any modifications to the protocol?:
 
Notes:
 
References: