| Cell Line Species/Tissue: |
human
/
acute monocytic leukemia |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
4 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
24% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Efficiency determined by counting X-gal stained cells. 350,000 cells were plated in 1.2 ml medium.
|
| References: |
| |