| Cell Line Species/Tissue: |
Human
/
histiocytic lymphoma, macrophage |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
no information |
Number of Cells:
|
no information |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
8 µl |
Amount of Reagent (µl):
|
25 µl |
Complex Incubation on Cells (hrs):
|
30 hrs |
Analysis Performed Post-Transfection (hrs):
|
30 hrs |
Transfection Efficiency (%):
|
350,000 RLU |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Split cells day prior to transfection. Tested DNA:Effectene ratios of 1:25 (best) & 1:50. Also tested SuperFect and achieved 75,000 RLU. |
| References: |
| |