Transfection Cell Database - Detailed View


Cell Line: NIH/3T3

Cell Line Species/Tissue: Mouse / Embryo fibroblast 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
b-gal , pCMV-beta 
Plasmid Purification Method:
EndoFree Plasmid Kit 
Plate Format:
96-well plate 
Number of Cells:
 
Percent Confluence(%):
60-80% 
Amount of Nucleic Acid (µg):
0.1 
Amount of Enhancer (µl):
0.8 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
297 beta-gal units/ml 
 
Any modifications to the protocol?:
 
Notes:
Incubated transfection complexes for up to 20h without increase in toxicity.  
References: