QIAxcel Advanced System

For effortless DNA and RNA analysis

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✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

QIAxcel Advanced Instrument

Cat. No. / ID: 9001941

Capillary electrophoresis device: delivered with computer, QIAxcel ScreenGel Software, and 1-year warranty on parts and labor

InstrumentSoftware

QIAxcel Advanced

QIAxcel ScreenGel Software

The QIAxcel Advanced Instrument has been discontinued. QIAGEN will maintain a stock and inventory of repair and spare parts for the QIAxcel Advanced Instrument, including providing associated repair services until December 31, 2029.

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The QIAxcel Advanced instrument is intended to be used only in combination with QIAxcel Kits for the applications described in the respective QIAxcel Kit handbooks.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The QIAxcel Advanced system replaces traditional, labor-intensive gel analysis of DNA and RNA by fully automating sensitive, high-resolution capillary electrophoresis of up to 96 samples per run. The QIAxcel ScreenGel software ensures convenient analysis and documentation of data.

The QIAxcel Advanced system operates in conjunction with the QIAxcel DNA Kits and QIAxcel RNA QC Kit v2.0

An upgraded QIAxcel instrument is now available. Do quality control of NGS libraries and liquid biopsy with the high-sensitivity capabilities of the QIAxcel Connect. Learn more at www.qiagen.com/qiaxcel-connect-system

Software

QIAxcel ScreenGel software provides a new level of convenience

QIAxcel ScreenGel software, specifically developed for use with the QIAxcel Advanced system, is a powerful and user-friendly tool for data collection and analysis. Interactive tools simplify analysis, facilitate rapid data interpretation, and provide flexibility with data and results displayed in both electropherogram and gel image formats. Results can be viewed individually or displayed as overlay views for sample and data comparison. All-in-one analysis for multiple data sets simplifies evaluation. A unique algorithm calculates and generates a tabular display of a variety of peak properties, including number of peaks as well as the size, height, width, and area of each peak. Comprehensive data reports can be easily generated and saved or exported to meet individual documentation needs. Complete process profiles for standardized sample processing — from running samples to data analysis, generating reports and exporting data — minimize the need for additional training for users.

QIAxcel ScreenGel software includes specific features that support the technical requirements of 21 CFR Part 11 regulations. Security features such as the following are available:

Password-protected login to prevent unauthorized access and data manipulation
Audit trail documentation for configuration files and system events
Automatic saving and archiving of write-protected raw data
Secure user management

Different user profiles are available (Routine, Basic, Advanced, Admin) and are password protected (user login is required) for increased security. A simplified interface minimizes the need for user training and makes the software especially attractive for inexperienced users.

Services

Are you thinking of upgrading to the latest QIAxcel generation, the QIAxcel Connect?

Then QIAGEN’s trade-in/trade-up program is just right for you!

QIAGEN makes it easy to keep up to speed with the latest automation and sample processing technologies. Simply trade up your old QIAxcel Advanced to the QIAxcel Connect, or trade in a competitor instrument to the QIAxcel Connect.

Learn more about trade-in/trade-up opportunities.

Resources

For calibration of signal intensity

For optimal DNA size determination

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For optimal RNA fragment size determination

For automated quantitative and qualitative RNA analysis using the QIAxcel and QIAxcel Advanced instruments

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

For optimal use of the QIAxcel Gel Cartridges

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

This software may only be downloaded by registered users with a valid QIAxcel ScreenGel Software license. If you do not have a valid software license, contact your QIAGEN sales representative.

These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

A protocol for evaluating quality of RNA samples using the QIAxcel Advanced Instrument and ScreenGel software version 1.3 or higher

Addressing critical factors and new solutions

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Method file for use with QIAxcel ScreenGel Software

Alignment marker file QX15/600 bp - English (XAM)

Method file for use with BioCalculator Software

Method file for use with QIAxcel ScreenGel Software

Process Profile and Method file for use with QIAxcel ScreenGel Software

Method file for use with BioCalculator Software

The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.

For effortless DNA and RNA analysis

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

For easy upgrade of QIAxcel ScreenGel Software Version 1.6.

FAQ

The QIAxcel System or QIAxcel Advanced works on the basis of fluorescence detection technology. Each channel has a separate excitation light source, with its own optical detection. So each gel cartridge channel is different in the fluorescence emission signal collection mode with its own background and/or baseline noise. When you use a new cartridge, calibration (intensity normalization) is necessary to equalize the variations.

FAQ ID -9019

First, check that the glass capillaries at the bottom of the cartridge are intact. You should see approximately 1 mm of glass capillary. Second, check that each tube has at least 10 µl sample volume.

Check the capillary is unblocked by performing the gel-droplet test as outlined in Appendix D in the QIAxcel DNA handbook.

Perform a detector test.

If the issue persists, please send data to QIAGEN Technical Service. The instructions for QIAxcel data folder can be found in FAQ ID-9008.

FAQ -9015

If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.

FAQ ID -1834

The solutions used with the QIAxcel System or QIAxcel Advanced should not have to be changed for the life of the Gel Cartridge (based on the number of runs). However, if any contamination is suspected, the Buffer Tray should be cleaned and the buffers should be changed immediately.

FAQ ID -1840

The minimum sample volume required for the QIAxcel System and QIAxcel Advanced is 10 ul to guarantee sample injection in each channel.

FAQ ID -1852

Yes, the QIAxcel System or QIAxcel Advanced can be connected to a local network.

FAQ ID -1862

The QIAxcel Advanced has been discontinued as of October 2022. This follows the launch of the QIAxcel Connect instrument, the next generation of the QIAxcel technology. QIAGEN will maintain a stock and inventory of repair and spare parts for the QIAxcel Advanced including providing associated repair services until December 31, 2029. Please contact your customer care or sales representative if you have any questions about the discontinuation of QIAxcel Advanced.

FAQ ID - 3983

The sample DNA concentration might be too low. You can increase sample injection time.

A very strong sample in the same run can cause the threshold to be set too high. As a result, the signal from weaker samples is below this threshold. Try diluting the sample with high concentration with the QX dilution buffer.

High salt concentration in the samples, such as restriction enzyme digested DNA, can interfere with sample injection. Try reducing the salt concentration by diluting such samples with the QX dilution buffer.

If the issue persists, please send data files to QIAGEN Technical Services. See <a href="/knowledge-and-support/faq/?ID=71818D37-21EF-40B0-BA30-6C8CC8CA82B9"><b>Where are the QIAxcel data stored</a></b>.

FAQ -9013