QIAsymphony DNA Kits

For automated purification of DNA from 1–96 samples

Products

QIAsymphony DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

QIAsymphony DNA Midi Kit (96)

Cat. No. / ID: 931255

For 96 preps of 1000 µl each: includes 2 reagent cartridges and enzyme racks and accessories

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QIAsymphony DNA Mini Kit (192)

Cat. No. / ID: 931236

For 192 preps of 200 µl each: includes 2 reagent cartridges and enzyme racks and accessories

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Features

Purification of DNA from a broad range of sample types
Novel, prefilled reagent cartridges for ease of use
High reproducibility through standardized processing
Bar codes enable tracking of samples and reagents

Product Details

QIAsymphony DNA Kits enable automated purification of DNA from a broad range of sample types on the QIAsymphony SP with sample volumes of up to 1 ml. Genomic DNA can be purified from human whole blood, buffy coat, human and animal tissues, formalin-fixed paraffin-embedded tissue (FFPE), cultured cells, and bacterial cultures. The QIAsymphony DNA Mini Kit also enables purification of viral nucleic acids from human whole blood. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Performance

Automated purification of DNA on the QIAsymphony SP provides high yields of DNA (see figure ""). Spectrophotometric analysis showed that the DNA was highly pure (see figure ""). DNA gave high-performance results in downstream PCR of a single-copy gene with no inhibition of PCR observed with 1 µl or 10 µl eluate in the amplification reaction (see figures "" and "").

See figures

High yields of DNA from human whole blood.

Highly pure DNA.

High-performance results in PCR — A.

High-performance results in PCR — B.

Principle

QIAsymphony DNA Kits combine the speed and efficiency of silica-based purification of genomic DNA with the convenient handling of magnetic particles (see figure ""). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure ""). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.

See figures

Efficient processing of magnetic particles.

Prefilled, sealed reagent cartridges.

Procedure

For tissues, cultured cells, and bacteria protocols, manual sample pretreatment is required. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.

QIAsymphony DNA protocols
Protocol	Kit	Sample material	Processed volume
DNA Blood 200	QIAsymphony DNA Mini Kit (192)	Human whole blood	200 μl
DNA Blood 400	QIAsymphony DNA Midi Kit (96)	Human whole blood	400 μl
DNA Blood 1000	QIAsymphony DNA Midi Kit (96)	Human whole blood	1000 μl
DNA Buffy Coat 200	QIAsymphony DNA Mini Kit (192)	Buffy coat	200 μl
DNA Buffy Coat 400	QIAsymphony DNA Midi Kit (96)	Buffy coat	400 μl
Tissue LC 200	QIAsymphony DNA Mini Kit (192)	Pretreated sample lysates from tissue, FFPE tissue, cultured cells, and bacterial cultures; for example: heart (25 mg), lung (25 mg), kidney (25 mg), muscle (50 mg), Jurkat cells (2 x 10⁶cells)*	200 μl
Tissue HC 200	QIAsymphony DNA Mini Kit (192)	Pretreated sample lysates from tissue, cultured cells, and bacterial cultures; for example: spleen (25 mg), liver (25 mg), Jurkat cells (1 x10⁷ cells), bacteria (4 x 10⁹ cells)*	200 μl
Virus Blood 200	QIAsymphony DNA Mini Kit (192)	Human whole blood	200 μl

* Values in brackets indicate the recommended maximum sample size.

Applications

DNA purified using QIAsymphony DNA Kits is of high quality and is ready for use in downstream applications, such as:

PCR and real-time PCR
Genotyping, STR, and SNP analysis
Sequencing

Supporting data and figures

High yields of DNA from human whole blood.

Total DNA was purified from human whole blood (3 donors, 6 replicates each) on the QIAsymphony SP using the QIAsymphony DNA Mini Kit (200 µl) or QIAsymphony DNA Midi Kit (1000 µl). Blood was collected in BD Vacutainer K2E tubes and stored at 5–8°C prior to use. Yield was determined by spectrophotometric analysis.

Specifications

Features	Specifications
Applications	PCR, Real-Time PCR, Genotyping
Sample type	Blood; Cell culture (only Mini Kit); Tissue (only Mini Kit)
Elution volume	Mini Kit: 50-200 µl; Midi Kit: 100-500 µl
Sample amount	Mini Kit: 200 µl blood, up to 50 mg tissue or up to 1 x 10e7 cells; Midi Kit: 400 µl or 1000 µl blood
Yield	Mini Kit: 4-10 µg from 200 µl blood; Midi Kit: 8-20 µg from 400 µl blood or 15-50 µg from 1000 µl blood

Resources

PAXgeneSaliva400 and PAXgeneSaliva1000 (supplementary for QIAsymphony DNA Midi Kit) QIAsymphony SP Protocol Sheet, R1, version 1

Sample and eluate tubes/racks that can be used with the QIAsymphony DNA Midi Kit, PAXgene Saliva Collector and QIAsymphony SP (Software version 5.0 ; labware package SOW-912-0-01)

For use with software version 4.0 or higher

For use with the QIAsymphony SP/AS instruments (software version 4.0)

For use with software version 5.0 or higher

FAQ

No. Modified QIAGEN protocols or custom protocols for QIAsymphony SP can be developed to meet your specific requirements and purchased from QIAGEN.

FAQ ID -1904

Yes. Any number of samples between 1 and 96 can be processed on the QIAsymphony SP.

FAQ ID -1906

No. We do not recommend use of carrier RNA with the QIAsymphony Virus Blood 200 Protocol.

FAQ ID -2163

In general, magnetic particles are not carried over into QIAsymphony eluates. However, if high amounts of genomic DNA are purified (large number of white blood cells in the sample) magnetic-particle carryover may be observed. If carryover does occur, magnetic particles in eluates will not affect most downstream applications. If magnetic particles need to be removed before performing downstream applications, tubes or plates containing eluates should first be placed in a suitable magnet and the eluates transferred to a clean tube.

FAQ ID -2166

Eluates obtained on the QIAsymphony SP can be stored at 2–8°C for 3 days before use in downstream applications and should be placed at –20°C or –80°C for long-term storage.

FAQ ID -2165

DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. Approximately 70–80 µg DNA can be isolated from 25 mg of spleen, 40–50 µg from 25 mg liver, and 10–15 µg from 50 mg muscle.

FAQ ID -1919

200–1000 µl sample volumes can be processed on the QIAsymphony Sample Prep Module.

FAQ ID -1907

The maximum amount of tissue that can be processed on the QIAsymphony SP using QIAsymphony DNA Mini Kit is 50 mg, and the maximum number of cultured cells is 1 x 10⁷.

See details for upper limits of various starting materials in the table below:

Sample type	Sample amount	Protocol
Heart	25 mg	Tissue Standard
Spleen	25 mg	Tissue High Content
Lung	25 mg	Tissue Standard
Liver	25 mg	Tissue High Content
Kidney	25 mg	Tissue Standard
Muscle	50 mg	Tissue Standard
Mouse tail	0.8 cm	Tissue Standard
Jurkat Cells	2 x 10e6	Tissue Standard
Jurkat Cells	1 x 10e7	Tissue High Content

FAQ ID -1917

A total of 192 samples can be processed with one QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol (96 samples per reagent cartridge).

FAQ ID -2159

Sample volumes of 200 µl can be processed with the Virus Blood 200 Protocol using the QIAsymphony DNA Mini Kit.

FAQ ID -2156

After pretreatment, 220 µl of the lysates must be transferred to the QIAsymphony SP to ensure that 200 µl of sample is processed.

FAQ ID -2992

Lysis time varies depending on the tissue type processed. For recommended lysis times refer to the tissue protocol sheets. If lysis is incomplete after recommended lysis time, as indicated by the presence of insoluble material or highly viscous lysates, lysis time can be prolonged or insoluble material can be removed by centrifugation. Overnight lysis is possible and does not affect the preparation.

FAQ ID -2993

Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using QIAsymphony DNA Kits.

Samples are lysed by Proteinase K digestion in lysis buffers optimized for tissue, cultured cells, and bacteria, respectively. For lysis of gram-positive bacteria, lysozyme is used in addition to Proteinase K. Lysed samples are homogenized by pipetting up and down, and insoluble material is removed by centrifugation (see the QIAsymphony DNA Handbook for details).

FAQ ID -1916

Samples should be processed on the QIAsymphony within 24 hours of collection. Samples should be transported and stored at 2–25°C.

FAQ ID -2157

Human whole blood samples treated with EDTA or citrate can be processed using the QIAsymphony Virus Blood 200 protocol with the QIAsymphony DNA Mini Kit. We do not recommend using heparin-treated blood samples.

FAQ ID -2154

Yes. The DNA Tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.

FAQ ID -1921

Yes. QIAsymphony DNA Investigator Kit protocols purify both genomic and mitochondrial DNA.

FAQ ID -2030

Final elution volumes of 60 µl, 85 µl, 110 µl, or 165 µl can be selected using the QIAsymphony Virus Blood 200 protocol. Please note that the initial elution volumes are 95 µl, 120 µl, 145 µl, or 200 µl. When calculating the amount of internal control(s) as well as the titer of the processed sample, it is necessary to take into consideration the initial volume of elution buffer that is used for each sample.

FAQ ID -2160

No. Protocols on the QIAsymphony SP cannot be modified by users. However, protocols allow elution volumes to be selected within a predefined range. Customized protocols for QIAsymphony SP will be available on request.

FAQ ID -1903

The High Content protocol for DNA extractions on the QIAsymphony SP is optimized for samples with an expected DNA yield of more than 30 µg. Up to 90 µg DNA can be purified from tissues with high cell densities (e.g., spleen) or large numbers of cultured cells.

FAQ ID -1918

Depending on the application, elution volumes on the QIAsymphony SP range from 30 µl to 500 µl.

FAQ ID -1908

Various types of tissue, cultured cells, and bacterial pellets can be processed using the tissue protocols for the QIAsymphony DNA Kits on the QIAsymphony SP.

FAQ ID -1915

No. The use of internal controls is not mandatory for use with the QIAsymphony Virus Blood 200 Protocol. If an internal control is not required, Buffer ATE without internal control is added to the samples. Buffer ATE must be placed in slot A of the sample drawer.

FAQ ID -2162

Yes. The DNA tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.

FAQ ID -2991

The target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony is viral DNA from released as well as cell-associated viruses, co-purified with genomic DNA from blood cells.

FAQ ID -2158