RNeasy DSP MinElute Cleanup Kit

For manual cleanup and concentration of total RNA for use with in-vitro diagnostic QIAGEN products where the RNeasy DSP MinElute Cleanup Kit is listed as an accessory

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RNeasy DSP MinElute Cleanup Kit (50)

Cat. No. / ID:   78244

50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
3.293,00 PLN
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The RNeasy DSP MinElute Cleanup Kit is intended for in vitro diagnostic use.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Concentration of small amounts of RNA to only 10 µL
  • High-quality RNA in a few minutes
  • RNA purification system for use with other QIAGEN IVD products

Product Details

The RNeasy DSP MinElute Cleanup Kit provides a manual procedure for cleanup and concentration of RNA using specialized RNeasy MinElute spin columns based on silica-membrane technology. For use with in-vitro diagnostic QIAGEN products that list the RNeasy DSP Cleanup  Kit as an accessory.

Principle

The RNeasy DSP MinElute Cleanup Kit provides fast and easy methods for cleanup and concentration of pre-purified RNA.

Procedure

The RNeasy DSP MinElute Cleanup Kit uses a simple bind–wash–elute procedure. A guanidine isothiocyanate-containing lysis buffer and ethanol are added to the sample to create conditions that promote selective binding of RNA to the RNeasy MinElute membrane. The sample is then applied to the RNeasy MinElute spin column. RNA binds to the silica membrane, contaminants are efficiently washed away, and pure concentrated RNA is eluted in a small volume of RNAse-free water. Detailed protocols can be found in the instructions for use of the corresponding downstream applications.

Applications

RNA purified with the RNeasy DSP MinElute Cleanup Kit can directly be used for sensitive analytical detection in downstream assays, such as qPCR. The specific procedure can be found in the instructions for use of the corresponding downstream applications.

Specifications

FeaturesSpecifications
ApplicationsDownstream detection procedures in molecular diagnostics, such as PCR
Elution volume10–14 µL
FormatMinElute spin column
Sample typeprepurified RNA
ProcessingManual (centrifugation)
TechnologySilica technology
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinTotal RNA

Resources

Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)