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EZ1&2 Virus Mini Kit v2.0 (48)
Cat. No. / ID: 955134
For 48 preps: Reagent Cartridges (Virus Mini v2.0), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer AVE, carrier RNA
The EZ1&2 Virus Mini Kit v2.0 is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Simultaneous purification of viral DNA and RNA
- Higher analytical sensitivity, even with low viral titers
- Highly reproducible results
- Linear yields from a wide range of viral titers
- Prefilled, sealed reagent cartridges for easy handling
Product Details
The EZ1&2 Virus Mini Kit v2.0 provides a fully automated procedure for simultaneous purification of viral DNA and RNA from serum, plasma, CSF, urine, respiratory samples, and other cell-free body fluids for sensitive analytical detection in downstream assays. The kit provides optimized binding conditions for more robust and reproducible capture of nucleic acids, and improved wash conditions for higher analytical sensitivity in downstream assays.
The EZ1 DSP Virus Kit is also available for viral nucleic acid purification for in vitro diagnostics (not available in all countries).
The EZ1&2 Virus Mini Kit v2.0 is automated with the EZ2 Connect (1–24 samples per run). This kit can also be used with the EZ1 Advanced XL (1–14 samples per run).
Principle
Automated purification with the EZ1&2 Virus Mini Kit v2.0 requires use of one of the following:
- the EZ2 Connect
- the EZ1 Advanced XL and the EZ1 Advanced XL Virus Card v2.0
EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.
Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Prefilled and sealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases.
Procedure
Serum or plasma samples (100 µl, 200 µl, or 400 µl) are loaded onto the instrument, and viral nucleic acids are purified in one step following the automated EZ1&2 Virus procedure. Viral nucleic acids are eluted in a volume of 150 µl, 120 µl, 90 µl or 60 µl and are ready to use in downstream applications.
The instrument makes viral nucleic acid purification convenient and reliable. The EZ1&2 Virus Mini Kit v2.0 provides prefilled, sealed reagent cartridges that help to prevent human error during setup of the instrument.
Specifications
| Features | Specifications |
|---|---|
| Applications | PCR, real-time RT-PCR |
| Elution volume | 50–200 µl |
| Technology | Magnetic particles |
| For automated processing | EZ2 Connect instruments or EZ1 Advanced XL |
| Sample amount | 100 µl, 200 µl, 400 µl |
| Processing | Automated |
| Format | Sample tube |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral DNA, viral RNA |
| Time per run or per prep | 40 minutes |
| Main sample type | Serum, plasma |
Resources
Quick-Start Protocols (3)
Kit Handbooks (2)
Brochures & Guides (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
FAQ
Is training of lab personnel included with the purchase of a BioRobot EZ1?
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
Where can I find information about the worktable setup on the BioRobot EZ1?
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
What disposables are required for nucleic acid isolations on the BioRobot EZ1?