Klenow Fragment (2500 U)
2500 U of Klenow Fragment (0.5 mL at 5000 U/mL) and 10x Blue Buffer (1 x 1.5 mL)
DNA Polymerase I (5000 U)
5000 U of DNA Polymerase I (10,000 U/mL) and 10x Blue Buffer. Storage temperature: –25°C to –15°C
Terminal Deoxynulceotidyl Transferase (TdT) (6000 U)
6000 U of Terminal Deoxynucleotidyl Transferase (TdT) (0.30 mL at 20,000 U/mL) and 10x Green Buffer (1 x 1.5 mL) and 2.5 mM Cobalt Chloride (1 x 1.5 mL)
T4 DNA Ligase (Rapid)
240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer
Poly(A) Polymerase (1000 U)
1000 U of Poly(A) Polymerase (5000 U/mL) and 10x Poly(A) Polymerase Reaction Buffer.
T7 DNA Ligase (900,000 U)
900,000 U of T7 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer
T4 Polynucleotide Kinase (10,000 U)
10,000 U T4 Polynucleotide Kinase (1.0 mL at 10,000 U/mL) and 10X Polynucleotide Kinase Buffer (2 x 1.5 mL)
AllTaq PCR Core Kit (250 U)
50 µl AllTaq Polymerase (5 U/µl), 1.2 ml AllTaq PCR Buffer (5x), 55 µl dNTP Mix (10 mM each), 200 µl Template Tracer (25x), 50 µl Master Mix Tracer (125x), 2 ml Q-Solution (5x), 1.2 ml MgCl2 (25 mM), 1.9 ml RNase-Free Water
Taq DNA Polymerase (250 U)
250 units Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2
RNase A (17,500 U)
2.5 ml (100 mg/ml; 7000 units/ml, solution)
Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.
HotStarTaq DNA Polymerase (250 U)
250 units HotStarTaq DNA Polymerase, 10x PCR Buffer, 5x Q-Solution, 25 mM MgCl2
Omniscript RT Kit (50)
For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1.1 ml RNase-free water
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps
QIAGEN Protease (7.5 AU)
7.5 Anson units per vial (lyophilized)
QIAGEN Proteinase K (2 ml)
2 ml (>600 mAU/ml, solution)