EZ1&2 DNA Tissue Kit

For automated purification of high-quality DNA from 1–24 samples per run

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EZ1&2 DNA Tissue Kit (48)

Cat. No. / ID: 953034

For 48 preps: Reagent Cartridges (Tissue), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer G2, Proteinase K

The name of this kit was changed from "EZ1 DNA Tissue Kit" to "EZ1&2 DNA Tissue Kit" to demonstrate that they are compatible with the new EZ2 Connect instruments as well as the EZ1 Advanced XL. No changes were made to the well-established kit technology.

The EZ1&2 DNA Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Standardized processing of up to 40 mg tissue samples
Rapid purification of up to 28 µg high-quality DNA
Flexible purification from a wide range of tissue types
Prefilled, sealed reagent cartridges for easy handling

Product Details

The EZ1&2 DNA Tissue Kit contain all required reagents and labware for rapid, automated purification of DNA from up to 40 mg tissue samples using magnetic-particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.

Protocols for automated purification of genomic DNA from a wide range of sample types using the EZ1&2 DNA Tissue Kit are provided in the handbook. These include buccal cells, cultured cells, dried blood and paraffin-embedded tissue sections.

The EZ1&2 DNA Tissue Kit are automated with the EZ2 Connect (1-24 samples per run). These kits can also be used with the EZ1 Advanced XL (1–14 samples per run).

Performance

DNA purified using EZ1&2 DNA Tissue Kit provides high-performance results in downstream applications, such as PCR (see figure “Consistent PCR results”).

See figures

Consistent PCR results.

Principle

Automated purification with EZ1&2 DNA Tissue Kit requires use of either EZ2 Connect instruments or EZ1 instruments and the EZ1 Advanced XL DNA Tissue Card.

EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.

Procedure

Tissues are first incubated in Buffer G2 containing proteinase K until lysed and then homogenized. Pretreated tissues are loaded onto the instrument. Genomic DNA is then purified in one step following the automated EZ1&2 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl or 50 µl volumes, ready to use in all downstream applications, such as PCR.

Applications

The high-quality DNA obtained using EZ1&2 DNA Tissue Kit is suited for use in many applications, such as:

Population genetics and pharmacogenomic research
PCR and real-time PCR
Sequencing
Oncology research
Genetic identity testing and forensics
Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies

Specifications

Features	EZ1&2 DNA Tissue Kit
Applications	PCR, real-time RT-PCR, genotyping
Elution volume	50, 100 and 200 µl
For automated processing	EZ2 Connect, EZ1 Advanced XL
Format	Sample tube
Main sample type	Tissue samples
Processing	Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	DNA
Sample amount	10–40 mg
Technology	Magnetic particles
Time per run or per prep	40 minutes
Yield	9–28 µg

Supporting data and figures

Consistent PCR results.

DNA was purified from various tissues using the EZ1&2 DNA Tissue procedure. [A] PCR of the bovine obesity single-copy gene (850 bp fragment) using EZ1-purified DNA from bovine tissues. [B] PCR of GAPDH (266 bp fragment) using EZ1-purified DNA from 6 mouse tails. A 2.5 µl aliquot (5%) of each PCR was visualized by agarose gel electrophoresis. M: 100 bp marker; –C: negative control.

Resources

For use with EZ2 instruments

For use with EZ1 instruments

EZ1&2 DNA Tissue Kit
For automated purification of DNA from tissue and other samples using EZ1 instruments

For automated purification of DNA from tissue and other samples using EZ2 Connect instruments

Fully automated low- to medium-throughput purification of nucleic acids

FAQ

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

Yes, please follow the Supplementary Protocol 'Isolation of DNA from cultured cells using the EZ1 DNA Tissue Kit' (MA15).

FAQ ID -990

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

Yes, please check the EZ1 DNA Tissue Handbook for instructions on pretreatment and extraction protocol.

FAQ ID -1260

For storage of DNA purified from forensic samples using the EZ1 DNA Tissue Kit in a buffered solution such as TE rather than water (used for elution in the EZ1 forensic protocols) we suggest the following:

Before storing the DNA, add 1/10 volume of 10x Forensic TE buffer (100 mM Tris·Cl, 1 mM EDTA, pH 8.3) to each sample. For example, add 5 µl of 10x Forensic TE buffer if you eluted in 50 µl. The DNA is ready to use, or can be stored at 2–8°C for 24 h, or at –20°C for longer periods.

FAQ ID -734

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

Bisulfite conversion of unmethylated cytosines into uracils with the EpiTect Bisulfite Kit works on DNA irrespective of the source organism. The DNA template needs to be of high purity for efficient conversion. We recommend to use genomic DNA extracted with our DNA isolation kits for clinical or animal and plant samples as a template for the EpiTect Bisulfite Kit.

FAQ ID -1209

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246