QIAamp DSP Circulating NA Kit

• Concentrated nucleic acids; high input, low elution volumes
• Efficient recovery of DNA and RNA
• No organic extraction or ethanol precipitation
• Removal of contaminants and inhibitors
• For in vitro diagnostic use

The QIAamp DSP Circulating NA Kit is for concentration and purification of free-circulating DNA and RNA from plasma for in vitro diagnostic use.

The QIAamp DSP Circulating NA Kit provides ease-of-use in diagnostic workflows, and is manufactured in accordance with GMP requirements. The procedure isolates and purifies high quality nucleic acids for use in downstream applications. The concentration of free-circulating nucleic acids in human blood plasma is usually low and varies considerably between individuals, ranging from 1–100 ng/ml in human samples. Nucleic acids are efficiently purified and concentrated from starting materials that contain low concentrations of mostly fragmented DNA and RNA (typically 1–100 ng/ml circulating DNA in human plasma).

The QIAamp DSP Circulating NA Kit simplifies isolation of circulating DNA and RNA from plasma samples by omitting phenol-chloroform extractions. Nucleic Acids bind specifically to the QIAamp Mini columns and contaminants pass through. PCR inhibitors are completely removed in the wash steps, leaving pure nucleic acids for elution with the provided buffer.

The QIAamp DSP Circulating NA Kit combines the selective binding properties of silica-based membrane with flexible elution volumes between 20 µl and 150 µl.

Tube extenders and vacuum processing on the QIAvac 24 Plus enable starting sample volumes of up to 5 ml (figure  Tube extenders for processing on the QIAvac 24 Plus). Flexible elution volumes between 20 µl and 150 µl allow concentration of nucleic acid species that are present in low concentrations.

The QIAamp DSP Circulating NA procedure includes 4 steps (lyse, bind, wash, and elute) that are carried out using QIAamp Mini columns on a vacuum manifold. The simple procedure is highly suited for simultaneous processing of multiple samples. Nucleic acids are efficiently purified and concentrated (figure  QIAamp DSP Circulating NA Kit procedure). Additionally, the QIAamp DSP Circulating NA Kit offers two distinct protocols: the Classic and Breeze protocols (figure  Comparison of Classic and Breeze workflows). The Classic protocol is for processing up to 5 ml plasma in 1 ml steps, and constitutes the unchanged protocol provided in the in the QIAamp DSP Circulating NA Kit Handbook, Version 1, Revision 3 (R3). The Breeze protocol is for processing up to 5 ml plasma in 1 ml steps, but has been optimized for low hands-on and turnaround time.

ccfDNA purified using the QIAamp DSP Circulating NA Kit is ready for use in a wide range of downstream applications, including NGS, RT-PCR, digital PCR, multiplex PCR and quantitative PCR.