TissueLyser Bead Dispensers

For dispensing beads into disruption vessels

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TissueLyser 5 mm Bead Dispenser, 96-Well

Cat. No. / ID: 69975

For dispensing 96 beads (5 mm diameter) in parallel

For

96 beads

Single beads

For beads

5 mm

3 mm

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✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Fast and efficient dispensing of grinding beads into the disruption vessels
Available for beads with different diameters
Compatible with TissueLyser systems

Product Details

TissueLyser Bead Dispensers are designed for efficient and user-friendly dispensing of grinding beads into disruption vessels for sample disruption using TissueLyser systems. TissueLyser Single-Bead Dispensers are for dispensing beads into standard 2 ml microcentrifuge tubes. The dispensers fit onto a Gilson Carrousel Pipette Stand. Dispensers are available for beads with diameters of 5 or 7 mm. TissueLyser Bead Dispensers, 96-Well are for dispensing 96 beads in parallel into Collection Microtubes (racked), enabling high-throughput disruption and homogenization. Dispensers are available for beads with diameters of 3 or 5 mm.

Resources

For dispensing beads into disruption vessels

For high-throughput disruption of biological samples

FAQ

Stainless steel beads sold by QIAGEN are not guaranteed to be magnetic. However, you may find some lots of the beads to be magnetic, due to small variations in the chemical compositions (which do not affect the disruptive functions of the beads).

FAQ ID -9180

The TissueLyser LT Adapter (69980) is not guaranteed to be airtight and we do not have an O-ring to seal the lid of the adapter.

If you are working with infectious agents, you will need to validate the disruption parameters on your own to make sure the pathogens do not escape outside.

You should find it possible to operate the TissueLyser LT in a laminar air flow hood.

FAQ ID -9116

TissueLyser LT Adapter is tolerant to temperatures between –78.5°C and +150°C.

Do not freeze the TissueLyser LT Adapter or the sample tubes in liquid nitrogen. The adapter and tubes may break and may cause injury.

However, the insert of the TissueLyser LT Adapter, the sample tubes, and grinding beads can be placed on dry ice for cooling prior to disruption.

FAQ ID -9111

Height: 280 mm

Width: 150 mm

Depth: 270 mm

FAQ ID -9114

Unfortunately, this is not possible. The 3 mm beads are not compatible with the 5 mm or 7 mm TissueLyser Single Bead Dispenser, as this could result in dispensation of multiple beads, and/or the dispenser may get blocked.

FAQ ID -9185

We are sorry, but we do not offer repair on the TissueLyser LT.

FAQ ID -9112

We recommend using 2 ml Sample Tubes RB (cat. no. 990381) with our TissueLyser LT.

FAQ ID -9113

We do not guarantee the stainless steel beads to be RNase free. However, when used according to our specifications for disruption of biological samples, no special treatment is needed to make the beads RNase free. This is because our chaotropic lysis buffers will usually inactivate these and RNases contained in the samples themselves.

FAQ ID -9181

Our stainless steel beads and tungsten carbide beads are ready to use.

FAQ ID -9183

When disrupting tough or very tough samples, we recommend using one and two 7 mm stainless steel beads, respectively, instead of one 5 mm stainless steel bead, to guarantee optimal disruption.

Do not exceed the recommended amount of tissue per sample tube. Please refer to the TissueLyser LT Handbook for our recommendations of starting sample amount for purification of DNA, RNA, or proteins from various sample types.

You may need to increase the time and/or intensity of homogenization from what is recommended in the TissueLyser LT Handbook. But please be aware that this may lead to some fragmentation of gDNA.

FAQ ID -9115

The 96-well TissueLyser Bead Dispenser can be cleaned with a mild detergent (e.g. dish-soap) and water. Do not use aggressive reagents such as acetone, and do not autoclave or heat-sterilize the TissueLyser Bead Dispenser.

FAQ ID -1055

The largest and most efficient beads that can be used with the TissueLyser LT are the 7 mm stainless steel beads. We suggest pre-disrupting teeth or bone into smaller pieces (e.g., using a hammer) and then use the resulting pieces for further disruption in the TissueLyser LT. In this case, we suggest cooling the samples and the adapter on dry ice prior to disruption, rather than disruption in a lysis buffer.

Please note that the adaptor and tubes should not be cooled using liquid nitrogen, as this could lead to breakage of the TissueLyser Adapter and the sample tubes and may cause injury.

FAQ ID -9110

Stainless steel beads can be reused for DNA extractions with DNeasy Plant Kits. Used beads can be recovered from cell-debris and cleaned using the procedure below:

Close the cap of the 2 ml microtube and briefly vortex to dislodge the bead and pellet from the bottom of the tube. If using grinding jars for the disruption of large sample volumes, skip to step 2.
Empty the contents of the tubes/jars into a sieve and rinse the beads thoroughly with water.
Incubate beads in 0.4 M HCl for 1 min at room temperature (15–25°C) to degrade any DNA and avoid cross-contamination in future preparations.
Rinse beads thoroughly with distilled water to remove the HCl.
Dry beads before use.

You can also find these instructions in Appendix B of the DNeasy Plant Handbook.

FAQ ID -9179