QIAxcel DNA Accessories

For use with QIAxcel capillary gel electrophoresis instruments for automated analysis of DNA samples

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QX Alignment Marker 15 bp/600 bp (1.5 ml)

Cat. No. / ID: 929530

Alignment marker with 15 bp and 600 bp fragments

Accessories

Marker

Plasticware

Instrument Accessories

Buffer

Type

Alignment

Size

Intensity Calibration

Fragment sizes

15 bp/600 bp

15 bp/1 kb

15 bp/3 kb

15 bp/5 kb

15 bp/10 kb

50 bp/1 kb

50 bp/5 kb

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QIAxcel DNA Accessories are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Accessories for automated capillary electrophoresis of DNA samples
For use with QIAxcel DNA Kits and a QIAxcel capillary electrophoresis instrument
Safety, convenience and a streamlined workflow

Product Details

QIAxcel accessories are used in combination with QIAxcel instruments for fully automated sensitive, high-resolution capillary electrophoresis of up to 96 samples per run.
The QIAxcel DNA Accessories only work with the QIAxcel DNA Kits which are mandatory components of the QIAxcel Connect System and its predecessor, the QIAxcel Advanced System.

Resources

For optimal DNA size determination

For calibrating migration time variation

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

For calibration of signal intensity

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Addressing critical factors and new solutions

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

These profiles are intended to be used to run and analyze samples together with the QX DNA Size Marker Large-Fragment Kit (SAP 929710) on the QIAxcel instrument and QIAxcel ScreenGel 1.6 Software.

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

FAQ

If lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly, open the channel of the sample that was incorrectly aligned. Check whether any unexpected extra peaks have been detected or if the alignment marker peaks do have any “shoulders” that are detected as separate peaks. Delete any extra peaks if necessary and re-analyze your data.

FAQ ID -1834

The concentration of the DNA marker, pUC18/HaeIII, provided in the QIAxcel DNA Kits is 5ug/ml in 1XTE buffer solution. Customers can run their own DNA size marker sample with different concentration levels using the provided Methods (i.e. M500, H500, L500 and so on).

The recommended method for all basic applications with the QIAxcel System is the M500 method. The BioCalculator software also allows customers to choose their own Sample Injection Time in the Sequence table. The M500 method provides 5KV for 20 seconds for the electro-kinetic injection of lower concentrated DNA samples. If the Injection Time is doubled or tripled (40 or 60 sec), you can expect double or triple intensities (also peak heights).

FAQ ID -1841

Data for each cartridge intensity calibration on the QIAxcel System is stored in a single file named 'calibration.log'. This file is saved in the BioCalculator root directory C:\Program Files\BioCalculator.

If, for any reason, a different computer is used than the one containing the calibration.log file, the file should be transferred to the new computer. Otherwise, recalibration of the cartridge is required.

Likewise, if the QIAxcel Gel cartridge is used on a different instrument than the one it was calibrated on, another intensity calibration should be performed.

FAQ ID -1825

If one of the capillaries of the gel cartridge for the QIAxcel System or QIAxcel Advanced is clogged, the corresponding lane in the gel-view image is typically black.

FAQ ID -1836