Which elution volume is used for the QIAsymphony DSP Circulating DNA Kit?
When an elution volume of 60 µl is selected, the instrument transfers 75 µl elution buffer to ensure that at least 60 µl eluate is available for downstream analysis. In most cases, the eluate volume is 60–70 µl, depending on temperature, humidity and how long the eluate remains on the instrument before being removed. To calculate total yield or recovery of an internal control, an elution volume of 75 µl should be used.