What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H-(P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?

EnzScript M-MLV Reverse Transcriptase RNase H– contains three point mutations that eliminate any measurable RNase H activity, an activity that is native to wild type M-MLV Reverse Transcriptase. Loss of RNase H activity enables greater yield of full length cDNA transcripts (>5kb) and increased thermal stability over wild type M-MLV Reverse Transcriptase (Gerard, G. F., et al. (2002) Nucl. Acids Res. 30:3118). Increased thermostability allows higher incubation temperatures of the first-strand reaction (up to 50°C), aiding in denaturation of template RNA secondary structure or GC-rich regions.