How much DNA should be loaded per well of an agarose gel?

The amount of DNA to load per well is variable. The least amount of DNA that can be detected with ethidum bromide is 10 ng. DNA amounts of up to 100 ng per well will result in a sharp, clean band on an ethidium bromide stained gel.  We recommend using the GelPilot Agarose for separation of amplified products using agarose gel electrophoresis.