What are the advantages of EnzScript M-MLV Reverse Transcriptase RNase H- (P7600) versus wild type M-MLV Reverse Transcriptase (P7040)?
EnzScript M-MLV Reverse Transcriptase (RT) RNase H- contains three point mutations that eliminate any measureable RNase H activity, which is native to wild type M-MLV RT. Loss of RNase H activity enables greater yield of full-length cDNA transcripts ( > 5kb) and increased thermal stability over wild type M-MLV RT*. Increased thermostability allows for higher incubation temperatures of the first-strand reaction (up to 50°C), aiding in denaturation of template RNA secondary structure or GC-rich regions.
*Reference:
Gerard G.F. et al., Nucl. Acids Res. (2002) 30 (14): 3118-3129
*Reference:
Gerard G.F. et al., Nucl. Acids Res. (2002) 30 (14): 3118-3129