Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?

No. HotStarTaq Plus DNA Polymerase is supplied with conventional QIAGEN PCR Buffer and CoralLoad PCR Buffer in separate vials. Both buffers minimize nonspecific amplification products, primer–dimers, and background.

CoralLoad PCR Buffer has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer.