| Cell Line Species/Tissue: |
Human adult dermal fibroblasts
/
|
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
Dulbebecco's modified Eagle's medium |
Percent Serum (%):
|
10% ftal bovine serum, gentamicin (100µg/ml), amphotericin B (100ng/ml) |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
no information |
Number of Cells:
|
|
Percent Confluence(%):
|
~70 % |
Amount of Nucleic Acid (µg):
|
no information |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
24-48 hrs |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
83% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| FOXO1 Functions as a Master Switch That Regulates gene Expression Necessary for Tumor Necrosis Factor-induced Fibroblast Apoptosis
Mani Alikhani, Zoubin Alikhani, and Dana T. Graves
JBC, Vol. 280, No. 13, Issue of April, pp. 12096-12102,2005
|