| Cell Line Species/Tissue: |
Mouse
/
Fibroblast |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
60-70 % |
Amount of Nucleic Acid (µg):
|
1,5 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
10 µl |
Complex Incubation on Cells (hrs):
|
4-6 h |
Analysis Performed Post-Transfection (hrs):
|
48 h |
Transfection Efficiency (%):
|
20-40 % |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |