| Cell Line Species/Tissue: |
Human
/
hematopoietic |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
2x10^5 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
4 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
85% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| An efficient gene transfer system for hematopoietic cell line using transient and stable vectors, Journal of Biotechnology 88 (2001) 159-165, Teixeira et al. |