| Cell Line Species/Tissue: |
Human
/
Fetal Liver |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM + 10%FBS+ antibiotics + glutamine |
Percent Serum (%):
|
10% |
Reporter System:
|
Luciferase |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
250000 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1,5µl |
Amount of Enhancer (µl):
|
3,2µl |
Amount of Reagent (µl):
|
10µl |
Complex Incubation on Cells (hrs):
|
48h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
50% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Very simple and no stress for cells
Compared Effectene to Electroporation.
Transfection efficency with electroporation: 30%
Transfection efficency with Effectene Reagent: 50%
|
| References: |
| |