Transfection Cell Database - Detailed View


Cell Line: NMuMG

Cell Line Species/Tissue: Human /  
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM High Glu 
Percent Serum (%):
10 % 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
12-well 
Number of Cells:
5x10e4/ml 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
640 ng 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
6 µl 
Complex Incubation on Cells (hrs):
48 h 
Analysis Performed Post-Transfection (hrs):
48 h 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 80~90%  
 
Any modifications to the protocol?:
None  
Notes:
Analysis method: Western Blot  
References: