| Cell Line Species/Tissue: |
Human
/
laryngeal epitheloid |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
MEM |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
96-well |
Number of Cells:
|
2x10*4 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.5, 0.1, 0.02 µM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
0.56 µl, 0.11 µl, 0.02 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48 h- 60h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
no information |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Antiviral Research 61 (2004) 195-206
Potent inhibition of respiratory syncitial virus by combination treatment with 2-5A antisense and ribavirin
Zan Xu et al. |