| Cell Line Species/Tissue: |
Human
/
fibroblast |
| Transfection Reagent: |
TransMessenger |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
12-well plates |
Number of Cells:
|
8x10*4 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
5 µl |
Amount of Enhancer (µl):
|
5 µl |
Amount of Reagent (µl):
|
5 µl |
Complex Incubation on Cells (hrs):
|
4 h |
Analysis Performed Post-Transfection (hrs):
|
12 h |
Transfection Efficiency (%):
|
60-70% |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Nitric Oxide 10 (2004)112 - 118
Analysis of cytokine-induced NO-dependent apoptosis using RNA interference or inhibition by 14o0W
Neil Beeharry et al. |