| Cell Line Species/Tissue: |
Rat
/
Brain, glioblastoma |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10% |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
100-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
80-90 |
Amount of Nucleic Acid (µg):
|
2 |
Amount of Enhancer (µl):
|
16 |
Amount of Reagent (µl):
|
60 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
40% |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| |
| References: |
| |