| Cell Line Species/Tissue: |
Rat
/
Brain, glioma |
| Transfection Reagent: |
RNAiFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM + 10% FCS |
Percent Serum (%):
|
10% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
10.000 |
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
1µg and 1,5µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
6µl and 9µl |
Complex Incubation on Cells (hrs):
|
24h |
Analysis Performed Post-Transfection (hrs):
|
24h |
Transfection Efficiency (%):
|
Not determinde |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| Complex formation in Buffer EC |
| Notes: |
| |
| References: |
| |