| Cell Line Species/Tissue: |
mouse
/
kidney |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM low glucose / glutamin/FCS |
Percent Serum (%):
|
10 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
cambio maxi prep |
Plate Format:
|
12-well plate |
Number of Cells:
|
3500 |
Percent Confluence(%):
|
30 |
Amount of Nucleic Acid (µg):
|
0.6 |
Amount of Enhancer (µl):
|
2.4 |
Amount of Reagent (µl):
|
6 |
Complex Incubation on Cells (hrs):
|
48 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
40 |
| |
| Any modifications to the protocol?:
|
| reduced enhancer |
| Notes: |
| |
| References: |
| |