Transfection Cell Database - Detailed View


Cell Line: Primary mouse fibroblasts

Cell Line Species/Tissue: mouse / fibroblasts 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 
Reporter System:
b-gal 
Plasmid Purification Method:
QIAGEN Plasmid Kit 
Plate Format:
12-well plate 
Number of Cells:
 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
1.2 
Amount of Enhancer (µl):
9.6 
Amount of Reagent (µl):
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
10-20% 
 
Any modifications to the protocol?:
 
Notes:
 
References: