| Cell Line Species/Tissue: |
Human
/
umbilical vein endothelial cells |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
EGM |
Percent Serum (%):
|
2 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
60-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
3 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
20 |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
70% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Transfection efficiency determined by X-gal staining. |
| References: |
| |