QIAamp MinElute Media Kit

For purification of DNA from liquid media

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QIAamp MinElute Media Kit

Cat. No. / ID: 57414

For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Proteinase K, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)

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The QIAamp MinElute Media Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Purification from a variety of liquid transport media
Time-saving vacuum procedure for convenient handling and ease of use
Flexible elution volumes from 20 to 150 µl
High-quality DNA with efficient removal of alcohols/contaminants

Product Details

The QIAamp MinElute Media Kit provides a convenient vacuum procedure for purification of nucleic acids from liquid media, such as cervical swab transport media. QIAamp MinElute columns are rapidly processed on QIAvac 24 Plus vacuum manifolds. Purification of DNA using the QIAamp MinElute Media Kit can be automated on the QIAcube Connect.

Performance

QIAamp MinElute Media Kit allows 250 µl liquid transport and storage media samples to be processed in <90 minutes. QIAamp MinElute columns processed on QIAvac 24 or QIAvac 24 Plus vacuum manifolds are processed in batches of 24 samples. DNA is eluted in 20–150 µl.

Principle

The QIAamp MinElute Media Kit uses well-established technology for purification of nucleic acids. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The kit is suitable for use with liquid media containing nucleic acids, such as cervical swab transport media (e.g., PreservCyt or SurePath solution). Nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Procedure

The QIAamp MinElute Media procedure comprises 4 steps (lyse, bind, wash, elute) and is carried out using QIAamp MinElute columns on QIAvac 24 Plus vacuum manifolds. The procedure is designed to ensure that there is no detectable sample-to-sample cross-contamination, and allows safe handling of potentially infectious samples. The simple QIAamp MinElute procedure, which is highly suited for simultaneous processing of multiple samples, yields pure nucleic acid from 24 samples in less than 90 minutes.

Applications

The QIAamp MinElute Media Kit can be used for isolation of DNA from liquid media, such as cervical swab transport media. The kit can be used for purification of cellular, bacterial, and viral nucleic acids from a variety of sources, including:

Liquid cytology media with alcohol (e.g., PreservCyt and SurePath)
Phosphate-buffered liquid transport media (e.g., M4RT)

Specifications

Features	Specifications
Applications	PCR, real-time PCR
Format	MinElute columns
Time per run or per prep	<90 minutes (24 samples)
Sample amount	250 µl
Elution volume	20–150 µl
Technology	Silica technology
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Viral DNA and RNA, bacterial DNA and RNA, cellular DNA and RNA
Yield	Varies
Main sample type	Liquid media
Processing	Manual (vacuum)

Resources

FAQ

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728