| Cell Line Species/Tissue: |
Homo sapiens
/
|
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
serumfree SFX medium |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
Endofree Plasmid Kit |
Plate Format:
|
96-well plate |
Number of Cells:
|
1000 |
Percent Confluence(%):
|
20 |
Amount of Nucleic Acid (µg):
|
0,66 |
Amount of Enhancer (µl):
|
5,3 |
Amount of Reagent (µl):
|
25 |
Complex Incubation on Cells (hrs):
|
48 |
Analysis Performed Post-Transfection (hrs):
|
36 |
Transfection Efficiency (%):
|
58 |
| |
| Any modifications to the protocol?:
|
| optimization beyond the suggestions in the handbook |
| Notes: |
| |
| References: |
| |