| Cell Line Species/Tissue: |
Drosophila Melanogaster
/
|
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
|
Reporter System:
|
none; assayed the expression level of rProtein |
Plasmid Purification Method:
|
Endofree Plasmid Kit, Qiagen mini |
Plate Format:
|
6-well plate |
Number of Cells:
|
3000000 |
Percent Confluence(%):
|
60% |
Amount of Nucleic Acid (µg):
|
1.2µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
4.5µl |
Complex Incubation on Cells (hrs):
|
16h |
Analysis Performed Post-Transfection (hrs):
|
2 weeks later after antibiotic selection |
Transfection Efficiency (%):
|
80% |
| |
| Any modifications to the protocol?:
|
| Yes |
| Notes: |
| I transfect the cells in suspension. Add the cells to the complexes mixes, mix well and plate 2 ml of cell/complex suspension into the wells. |
| References: |
| |