| Cell Line Species/Tissue: |
mouse
/
macrophage |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10 |
Reporter System:
|
Luciferase,b-gal |
Plasmid Purification Method:
|
QIAfilter Plasmid Kit |
Plate Format:
|
24-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
25-40 |
Amount of Nucleic Acid (µg):
|
0,25 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
5 |
Complex Incubation on Cells (hrs):
|
4 |
Analysis Performed Post-Transfection (hrs):
|
24-30 |
Transfection Efficiency (%):
|
25-50% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |