| Cell Line Species/Tissue: |
human
/
foreskin |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM with antibiotics |
Percent Serum (%):
|
10 |
Reporter System:
|
CAT,Luciferase,b-gal,GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
60-70 |
Amount of Nucleic Acid (µg):
|
0.2 to 0.4 |
Amount of Enhancer (µl):
|
1.6 |
Amount of Reagent (µl):
|
2 to 10 |
Complex Incubation on Cells (hrs):
|
2 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
1.5 to 8.8% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |