| Cell Line Species/Tissue: |
human
/
osteosarcoma bone, epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM + 10% foetal calf serum + pen/strep |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
60 |
Amount of Nucleic Acid (µg):
|
0.8 |
Amount of Enhancer (µl):
|
6.4 |
Amount of Reagent (µl):
|
8 |
Complex Incubation on Cells (hrs):
|
16 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
30-40 |
| |
| Any modifications to the protocol?:
|
| / |
| Notes: |
| |
| References: |
| |