| Cell Line Species/Tissue: |
canine
/
normal kidney, epithelial |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM + L-glutamine |
Percent Serum (%):
|
10 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
|
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 |
Amount of Enhancer (µl):
|
8 |
Amount of Reagent (µl):
|
10 |
Complex Incubation on Cells (hrs):
|
6 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
20-25% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Transfection efficiency determined by X-gal staining. |
| References: |
| |